試劑盒 > 酶聯免疫試劑盒 > 人葉酸(FA) ELISA Kit
    • ELISA Kit
    • 人葉酸(FA) ELISA Kit
  • 產品類型:
    ELISA Kit
  • 產品名稱:

    毕尔巴鄂巴斯克:人葉酸(FA) ELISA Kit

  • 英文名稱:

    Human Folic acid(FA) ELISA Kit

  • 貨號:
    CSB-E17109h
  • 別名:
    N/A
  • 規格:
    96T
  • 中文價格:
    3600
  • 種屬:
    Homo sapiens (Human)
  • 其他種屬:
  • 待測物名稱:
    Folic acid(FA)
  • 縮寫:
    FA
  • 蛋白功能1:
    Metabolism
  • 檢測范圍:
    12.5 pg/mL-200 pg/mL
  • 靈敏度:
    6.25 pg/mL
  • 反應時間:
    1-5h
  • 所需樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 下載:
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.093 0.090 0.092    
    100 0.227 0.208 0.218    
    50 0.378 0.367 0.373    
    25 0.618 0.637 0.628    
    12.5 0.986 1.003 0.995    
    0 1.485 1.429 1.457    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 89  
    Range % 84-93  
    1:40 Average % 93  
    Range % 89-97  
    1:80 Average % 95  
    Range % 90-100  
    1:160 Average % 84  
    Range % 80-88  
  • 回收率:
    The recovery of human FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-105  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 定性/定量:
    quantitative
  • 研究領域:
    Metabolism
  • 引用文獻:
    1. Serum homocysteine is associated with polycystic ovarian syndrome in Jordan , Nesreen Saadeh.et al,BIOMEDICAL REPORTS,2018
    2. Studying the association between methylene tetrahdrofolate reductase (MTHFR) 677 gene polymorphism, cardiovascular risk and lichen planus , Laila Rashed.et al,Journal of Oral Pathology&Medicine,2017
    3. Associations between homocysteine metabolism related SNPs and carotid intima-media thickness: a Chinese sib pair study , Sun K.et al,J Thromb Thrombolysis.,2016