試劑盒 > 酶聯免疫試劑盒 > 人免疫球蛋白A(IgA)ELISA Kit
    • ELISA Kit
    • 人免疫球蛋白A(IgA)ELISA Kit
  • 產品類型:
    ELISA Kit
  • 產品名稱:

    萨拉毕尔巴鄂:人免疫球蛋白A(IgA)ELISA Kit

  • 英文名稱:

    Human Immunoglobulin A,IgA ELISA Kit

  • 貨號:
    CSB-E07985h
  • 別名:
    N/A
  • 規格:
    96T
  • 中文價格:
    3600
  • 種屬:
    Homo sapiens (Human)
  • 其他種屬:
  • 待測物名稱:
    Immunoglobulin A,IgA
  • 縮寫:
    IgA
  • 蛋白功能1:
    Immunoglobulin
  • 檢測范圍:
    0.24 ng/mL-1000 ng/mL
  • 靈敏度:
    0.24 ng/mL
  • 反應時間:
    1-5h
  • 所需樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 下載:
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1000 2.180 2.205 2.193 2.124  
    250 2.093 2.002 2.048 1.979  
    62.5 1.841 1.868 1.855 1.786  
    15.6 1.528 1.501 1.515 1.446  
    3.9 0.772 0.753 0.763 0.694  
    0.98 0.396 0.397 0.397 0.328  
    0.24 0.189 0.180 0.185 0.116  
    0 0.069 0.068 0.069    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:10000 Average % 89  
    Range % 85-94  
    1:20000 Average % 90  
    Range % 86-97  
    1:40000 Average % 96  
    Range % 90-103  
    1:80000 Average % 89  
    Range % 86-93  
  • 回收率:
    The recovery of human IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 89-94  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 定性/定量:
    quantitative
  • 研究領域:
    Immunology
  • 引用文獻:
    1. Microbial, Biochemical & Immunological Assessment of Dental Caries , Devarasa Giriyapura Murugeshappa, et al,Annals of Medical and Health Sciences Research,2018
    2. Gluten-Related Disorders in a Population of Canadian Adults , Gesmundo I.et al,Proc Natl Acad Sci U S A,2017
    3. Prevalence of positive coeliac disease serology and HLA risk genotypes in a multiethnic population of adults in Canada: a cross-sectional study , Joseph Jamnik.et al,BMJ OPEN,2017
    4. ELISA TECHNIQUE FOR CLINICAL EVALUATION OF SERUM IMMUNOGLOBULIN-A IN IRAQI HYPERGLYCEMIA PATIENTS , Alhamd A K J.et al,WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES,2015