抗體 > 單克隆抗體 > CD146 Monoclonal Antibody
  • 產品類型:
    Monoclonal Antibody
  • 產品名稱:

    毕尔巴鄂竞技老板:CD146 Monoclonal Antibody

  • 貨號:
    CSB-MA013563A0m
  • 規格:
    ¥2,000
  • 種屬:
    Human
  • 免疫原:
    Recombinant Human Cell surface glycoprotein MUC18 protein (50-646AA)
  • 宿主:
    Mouse
  • 種屬反應性:
    Human
  • 應用范圍:
    ELISA, WB, IF, FC; Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200
  • 背景:
    Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    IgG2a
  • 純化方式:
    >95%, Protein G purified
  • 標記方式:
    Non-conjugated
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式:
    Liquid
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 圖片:

    毕尔巴鄂旅游攻略 www.wvrzzx.com.cn


    Western Blot

    Positive WB detected in: MCF-7 whole cell lysate

    All lanes: CD146 antibody at 1:1250

    Secondary

    Goat polyclonal to Mouse IgG at 1/50000 dilution

    Predicted band size: 72, 58 kDa

    Observed band size: 120 kDa



    Western Blot

    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate

    All lanes: CD146 antibody at 1:500

    Secondary

    Goat polyclonal to Mouse IgG at 1/50000 dilution

    Predicted band size: 72, 58 kDa

    Observed band size: 120 kDa



    Immunofluorescence staining of A375 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Immunofluorescence staining of Hela cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Immunofluorescence staining of MCF-7 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Immunofluorescence staining of THP-1 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Overlay histogram showing MCF-7 cells stained with CSB-MA013563A0m (red line) at 1:400. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

  • 說明書:

    注:產品說明書可能有改進,請直接與CUSABIO產品專員聯系,索取最新版說明書!

  • 別名:
    Cell surface glycoprotein MUC18 (Cell surface glycoprotein P1H12) (Melanoma cell adhesion molecule) (Melanoma-associated antigen A32) (Melanoma-associated antigen MUC18) (S-endo 1 endothelial-associated antigen) (CD antigen CD146), MCAM, MUC18
  • 克隆號:
    4D10C7