抗體 > 單克隆抗體 > CD14 Monoclonal Antibody
  • 產品類型:
    Monoclonal Antibody
  • 產品名稱:

    梅西毕尔巴鄂竞技:CD14 Monoclonal Antibody

  • 貨號:
    CSB-MA004879A0m
  • 規格:
    ¥2,000
  • 種屬:
    Human
  • 免疫原:
    Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)
  • 宿主:
    Mouse
  • 種屬反應性:
    Human, Mouse, Rabbit
  • 應用范圍:
    ELISA, WB, IHC, IF, FC; Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200
  • 背景:
    Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187).
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    IgG1
  • 純化方式:
    >95%, Protein A purified
  • 標記方式:
    Non-conjugated
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式:
    Liquid
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 圖片:

    毕尔巴鄂旅游攻略 www.wvrzzx.com.cn  


    Western Blot

    Positive WB detected in: Rabbit spleen tissue, Rabbit small intestine tissue

    All lanes: CD14 antibody at 1:2500

    Secondary

    Goat polyclonal to Mouse IgG at 1/10000 dilution

    Predicted band size: 41 kDa

    Observed band size: 55 kDa



    Western Blot

    Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate

    All lanes: CD14 antibody at 1:1800

    Secondary

    Goat polyclonal to Mouse IgG at 1/10000 dilution

    Predicted band size: 41 kDa

    Observed band size: 55 kDa



    IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.



    IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.



    IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.



    IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.



    IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.



    IHC image of CSB-MA004879A0m diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.



    Immunofluorescence staining of A549 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Immunofluorescence staining of Hela cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Immunofluorescence staining of HepG2 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Immunofluorescence staining of RAW264.7 cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Immunofluorescence staining of SY5Y cells with CSB-MA004879A0m at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).



    Overlay histogram showing Jurkat cells stained with CSB-MA004879A0m (red line) at 1:180. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.



    Overlay histogram showing RAW264.7 cells stained with CSB-MA004879A0m (red line) at 1:180. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

  • 說明書:

    注:產品說明書可能有改進,請直接與CUSABIO產品專員聯系,索取最新版說明書!

  • 別名:
    Monocyte differentiation antigen CD14 (Myeloid cell-specific leucine-rich glycoprotein) (CD antigen CD14) [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form], CD14
  • 指標名稱:
    CD14
  • 克隆號:
    14G1F3